nova red hrp substrate Search Results


nova red  (Vector Laboratories)
96
Vector Laboratories nova red
Nova Red, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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vectastain pk 6100 rabbit igg elite abc kit  (Vector Laboratories)
99
Vector Laboratories vectastain pk 6100 rabbit igg elite abc kit
Vectastain Pk 6100 Rabbit Igg Elite Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nova red substrate  (Vector Laboratories)
96
Vector Laboratories nova red substrate
Nova Red Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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hrp  (Vector Laboratories)
96
Vector Laboratories hrp
Vero cells were transfected with plasmid vectors expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of lipofectamine. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) (1:500 or 1:50) or <t>mouse</t> <t>anti-FLAG</t> (S-o) (1:2500 or 1:200) antibody and stained with either (A) <t>HRP</t> staining or (B) Alexa fluore 647 conjugated goat anti-mouse secondary antibody (1:1000). Cellular tubulin was stained with rabbit anti-alpha tubulin (abcam; 1:200) and anti-rabbit secondary antibody conjugated with alexa fluore 488. DAPI was used to stain nuclei of cells. Phase contrast images were taken at X10 magnification, whereas the fluorescent images were taken at 40X magnification.
Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
hrp - by Bioz Stars, 2026-06
96/100 stars
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nova red peroxidase substrate kits  (Vector Laboratories)
96
Vector Laboratories nova red peroxidase substrate kits
Vero cells were transfected with plasmid vectors expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of lipofectamine. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) (1:500 or 1:50) or <t>mouse</t> <t>anti-FLAG</t> (S-o) (1:2500 or 1:200) antibody and stained with either (A) <t>HRP</t> staining or (B) Alexa fluore 647 conjugated goat anti-mouse secondary antibody (1:1000). Cellular tubulin was stained with rabbit anti-alpha tubulin (abcam; 1:200) and anti-rabbit secondary antibody conjugated with alexa fluore 488. DAPI was used to stain nuclei of cells. Phase contrast images were taken at X10 magnification, whereas the fluorescent images were taken at 40X magnification.
Nova Red Peroxidase Substrate Kits, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
nova red peroxidase substrate kits - by Bioz Stars, 2026-06
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immpact nova red chromogen substrate  (Vector Laboratories)
95
Vector Laboratories immpact nova red chromogen substrate
Vero cells were transfected with plasmid vectors expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of lipofectamine. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) (1:500 or 1:50) or <t>mouse</t> <t>anti-FLAG</t> (S-o) (1:2500 or 1:200) antibody and stained with either (A) <t>HRP</t> staining or (B) Alexa fluore 647 conjugated goat anti-mouse secondary antibody (1:1000). Cellular tubulin was stained with rabbit anti-alpha tubulin (abcam; 1:200) and anti-rabbit secondary antibody conjugated with alexa fluore 488. DAPI was used to stain nuclei of cells. Phase contrast images were taken at X10 magnification, whereas the fluorescent images were taken at 40X magnification.
Immpact Nova Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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smooth muscle alpha actin ap vector blue vimentin  (Vector Laboratories)
96
Vector Laboratories smooth muscle alpha actin ap vector blue vimentin
Vero cells were transfected with plasmid vectors expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of lipofectamine. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) (1:500 or 1:50) or <t>mouse</t> <t>anti-FLAG</t> (S-o) (1:2500 or 1:200) antibody and stained with either (A) <t>HRP</t> staining or (B) Alexa fluore 647 conjugated goat anti-mouse secondary antibody (1:1000). Cellular tubulin was stained with rabbit anti-alpha tubulin (abcam; 1:200) and anti-rabbit secondary antibody conjugated with alexa fluore 488. DAPI was used to stain nuclei of cells. Phase contrast images were taken at X10 magnification, whereas the fluorescent images were taken at 40X magnification.
Smooth Muscle Alpha Actin Ap Vector Blue Vimentin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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immunoperoxidase system  (Vector Laboratories)
96
Vector Laboratories immunoperoxidase system
Vero cells were transfected with plasmid vectors expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of lipofectamine. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) (1:500 or 1:50) or <t>mouse</t> <t>anti-FLAG</t> (S-o) (1:2500 or 1:200) antibody and stained with either (A) <t>HRP</t> staining or (B) Alexa fluore 647 conjugated goat anti-mouse secondary antibody (1:1000). Cellular tubulin was stained with rabbit anti-alpha tubulin (abcam; 1:200) and anti-rabbit secondary antibody conjugated with alexa fluore 488. DAPI was used to stain nuclei of cells. Phase contrast images were taken at X10 magnification, whereas the fluorescent images were taken at 40X magnification.
Immunoperoxidase System, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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immpact (tm) novared (tm) hrp substrate  (Novus Biologicals)
N/A
ImmPACT (TM) NovaRED (TM) HRP Substrate
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Vero cells were transfected with plasmid vectors expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of lipofectamine. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) (1:500 or 1:50) or mouse anti-FLAG (S-o) (1:2500 or 1:200) antibody and stained with either (A) HRP staining or (B) Alexa fluore 647 conjugated goat anti-mouse secondary antibody (1:1000). Cellular tubulin was stained with rabbit anti-alpha tubulin (abcam; 1:200) and anti-rabbit secondary antibody conjugated with alexa fluore 488. DAPI was used to stain nuclei of cells. Phase contrast images were taken at X10 magnification, whereas the fluorescent images were taken at 40X magnification.

Journal: bioRxiv

Article Title: The anti-HIV Drug Nelfinavir Mesylate (Viracept) is a Potent Inhibitor of Cell Fusion Caused by the SARS-CoV-2 Spike (S) Glycoprotein Warranting further Evaluation as an Antiviral against COVID-19 infections

doi: 10.1101/2020.04.24.060376

Figure Lengend Snippet: Vero cells were transfected with plasmid vectors expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of lipofectamine. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) (1:500 or 1:50) or mouse anti-FLAG (S-o) (1:2500 or 1:200) antibody and stained with either (A) HRP staining or (B) Alexa fluore 647 conjugated goat anti-mouse secondary antibody (1:1000). Cellular tubulin was stained with rabbit anti-alpha tubulin (abcam; 1:200) and anti-rabbit secondary antibody conjugated with alexa fluore 488. DAPI was used to stain nuclei of cells. Phase contrast images were taken at X10 magnification, whereas the fluorescent images were taken at 40X magnification.

Article Snippet: Cells were stained for FLAG (S-o, anti-FLAG-1:2500) or N-myc (S-n, anti-myc-1:500) with HRP (Vector Nova Red kit) for phase contrast microscopy.

Techniques: Transfection, Plasmid Preparation, Expressing, Incubation, Staining

(A) Vero cells were transfected with plasmids expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of DMSO. (B) S-n and S-o glycoproteins were expressed as in A. Nelfinavir was added at the time of transfection at the concentrations indicated. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) or mouse anti-FLAG (S-o) antibody and stained with HRP staining followed by goat anti-mouse secondary antibody incubation. Images were taken at X10 magnification.

Journal: bioRxiv

Article Title: The anti-HIV Drug Nelfinavir Mesylate (Viracept) is a Potent Inhibitor of Cell Fusion Caused by the SARS-CoV-2 Spike (S) Glycoprotein Warranting further Evaluation as an Antiviral against COVID-19 infections

doi: 10.1101/2020.04.24.060376

Figure Lengend Snippet: (A) Vero cells were transfected with plasmids expressing either the S-o or S-n glycoproteins tagged with the 3xFLAG and N-myc epitopes at their amino termini, respectively. S-n and S-o expression was detected with mAbs against the epitope tags at 48 hours post transfection and compared to vehicle containing equivalent amount of DMSO. (B) S-n and S-o glycoproteins were expressed as in A. Nelfinavir was added at the time of transfection at the concentrations indicated. Methanol fixed cells were incubated with mouse anti-N-myc (S-n) or mouse anti-FLAG (S-o) antibody and stained with HRP staining followed by goat anti-mouse secondary antibody incubation. Images were taken at X10 magnification.

Article Snippet: Cells were stained for FLAG (S-o, anti-FLAG-1:2500) or N-myc (S-n, anti-myc-1:500) with HRP (Vector Nova Red kit) for phase contrast microscopy.

Techniques: Transfection, Expressing, Incubation, Staining